Journal of Genetic and Environmental Resources Conservation

Evaluation of In vitro antibacterial activity Vibrio cholera using silver nanoparticles synthesized from banana peel extract

Aleaa Abdulhussein Jameel , Raghad Alaa Baker — 2025-01-15

Recent advances in nanotechnology and the synthesis of nanoparticles through biosynthesis have increased the urge in scientists than for chemical or physical methods. The biosynthesis method is the most significant method than a conventional method because of its eco-friendly, low cost and rapid synthesizing process. The present study describes the antibacterial activity of silver nanoparticles (AgNPs) synthesized from banana peel extracts. The synthesis of AgNPs was confirmed by color change from light yellow to brown color. Further, the morphology of the biosynthesized nanoparticles, average size and presence of functional groups were characterized by UV – Visible spectroscopy (UV-Vis), X-Ray diffraction (XRD) and Fourier transform infrared spectroscopy, respectively. The UV spectra results show a strong resonance center and surface of silver nanoparticles at 450 nm. XRD studies revealed that the synthesized AgNPs show crystalline in shape. The FT-IR spectrum described the biological molecules which stabilize and form the silver nanoparticles in the aqueous medium. The antimicrobial property of AgNPs was tested against Vibrio cholerae, which showed maximum zones of inhibition of 32 mm at a concentration of 100 µL. Therefore, the biosynthesized AgNPs proved to have significant antibacterial activity.

Preserving and propropagation of the most important cherry and plum rootstocks introduced from Italy using plant tissue culture technique in Syria

Wafaa Koaym — 2025-01-15

Plant tissue culture has become an important technique in plant production. This research was carried out in the Laboratory of Biotechnology for Medicinal Plants of the National Commission for Biotechnology/Damascus, during the period between 2022-2023, to study the effect of some growth regulators on multiplication and rooting of the most important cherry rootstocks (Weiroot 10, Maxma 14) and clonal rootstock (Marianna GF8-1) Introduced From Italy and determine which combination of growth regulators lead to the highest rate of multiplication (in terms of number of shoots and length), and the best rooting (in terms of rooting percentage, number of roots and length). The highest survival and uncontaminated explants percentages were 82.26%, 70% and 66.5% obtained with 0.1% HgCl2 for 1 min in rootstocks Weiroot 10, Maxma 14 and Marianna GF8-1, respectively. The results indicated that Murashige and Skoog (MS) medium supplemented with 1.0 mg/l BA , 0.1 mg/l IBA and 0.2 mg/l GA3, achieved the highest shoot multiplication with an average of 4.85 and 4.62 shoots per explant and an average of 3.94 cm and 3.85 cm shoot length in rootstock Weiroot 10 and Maxma 14 respectively, and MS medium supplemented with 1.0 mg/l BA and 0.2 mg/l GA3, achieved the highest shoot multiplication with an average of 5.55 shoots per explant and an average of 3.62 cm shoot length in rootstock Marianna GF8-1. The greatest rooting percentages were 97%, 95% and 82.5% and the largest average number of roots were 5.12, 3.15 and 4.55 obtained when using IBA auxin at a concentration of 0.5 mg/l for studied rootstocks Maxma 14, Weiroot 10 and Marianna GF8-1, respectively with an average of 4.65 cm root length. The acclimatization percentages ranged between 74% and 91.5% in both rootstocks Marianna GF8-1 and Weiroot 10, respectively.

Estimation and analyzing sexual dimorphism for body weight and carcass traits of white and brown Domyati ducks

2025-02-15

The difference in body weight between males and females, hereafter called sexual dimorphism, the present study aimed to study the difference between white and brown Domyati duck for sexual dimorphism. At marketing age (8 wks) 16 (8 male + 8 female) brown and 14 white (7 male + 7 female) Domyati ducks, were used to estimate the body weight and carcass traits as non-edible meat parts (blood- feather- head- legs – viscera), edible meat parts (dressed carcass- liver- gizzard- heart), muscles (major – minor- breast muscles – thigh – drumstick), abdominal fat, gizzard fat, skin, wings, skeleton, and neck. Our results showed that the white Domyati ducks were significantly higher for sexual dimorphism for all traits compared to brown Domyati ducks. There was a significantly high positive correlation between body weight and abdominal fat, major, minor, breast muscles, thigh, and drumstick in male and female brown Domyati ducks. The same trend was noticed for white male and female Domyati ducks. However, the spleen had a lower positive correlation with body weight on both sexes of brown Domyati ducks, while, the spleen recorded a higher positive correlation with body weight of both sexes of white Domyati duck strain. In conclusion, there was a significant difference between white and brown Domyati ducks for some traits.